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Issue 15, 2013
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Dielectrophoresis based continuous-flow nano sorter: fast quality control of gene vaccines

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Abstract

We present a prototype nanofluidic device, developed for the continuous-flow dielectrophoretic (DEP) fractionation, purification, and quality control of sample suspensions for gene vaccine production. The device consists of a cross injector, two operation regions, and separate outlets where the analytes are collected. In each DEP operation region, an inhomogeneous electric field is generated at a channel spanning insulating ridge. The samples are driven by ac and dc voltages that generate a dielectrophoretic potential at the ridge as well as (linear) electrokinetics. Since the DEP potential differs at the two ridges, probes of three and more species can be iteratively fully fractionated. We demonstrate the fast and efficient separation of parental plasmid, miniplasmid, and minicircle DNA, where the latter is applicable as a gene vaccine. Since the present technique is virtually label-free, it offers a fast purification and in-process quality control with low consumption, in parallel, for the production of gene vaccines.

Graphical abstract: Dielectrophoresis based continuous-flow nano sorter: fast quality control of gene vaccines

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Publication details

The article was received on 16 Apr 2013, accepted on 21 May 2013 and first published on 22 May 2013


Article type: Paper
DOI: 10.1039/C3LC50475A
Citation: Lab Chip, 2013,13, 3111-3118
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    Dielectrophoresis based continuous-flow nano sorter: fast quality control of gene vaccines

    M. Viefhues, S. Wegener, A. Rischmüller, M. Schleef and D. Anselmetti, Lab Chip, 2013, 13, 3111
    DOI: 10.1039/C3LC50475A

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