Jump to main content
Jump to site search
Publishing
Advanced

Issue 18, 2013
Previous Article Next Article

Flow-enhanced electrochemical immunosensors on centrifugal microfluidic platforms

Tae-Hyeong Kim,a  Kameel Abi-Samra,ab  Vijaya Sunkara,a  Dong-Kyu Park,a  Mary Amasia,ab  Nahui Kim,c  Jintae Kim,c  Hanshin Kim,c  Marc Madou*ab  and  Yoon-Kyoung Cho*a 
Author affiliations

* Corresponding authors

a Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, South Korea
E-mail: ykcho@unist.ac.kr
Tel: +82 52 217 2511

b Department of Biomedical Engineering, University of California, Irvine, USA
E-mail: mmadou@uci.edu
Tel: +1 949 824 4143

c Health & Medical Equipment Business Team, Samsung Electronics, Suwon, South Korea

Abstract

We present a novel fully integrated centrifugal microfluidic device with features for target antigen capture from biological samples, via a bead-based enzyme-linked immune-sorbent assay, and flow-enhanced electrochemical detection. The limit of detection (LOD) of our device for the C-reactive protein (CRP) was determined to be 4.9 pg mL−1, a 17-fold improvement over quantification by optical density. The complete sample-to-answer protocol of our device is fully automated and takes less than 20 min. Overall, the presented microfluidic disc adds to the comparatively small number of fully integrated microfluidic-based platforms that utilize electrochemical detection and exemplifies how electrochemical detection can be enhanced by flow to successfully detect very low levels of biomarkers (e.g. pg mL−1).

Graphical abstract: Flow-enhanced electrochemical immunosensors on centrifugal microfluidic platforms

Back to tab navigation
Download options Please wait...

Publication details

The article was received on 22 Mar 2013, accepted on 23 Jun 2013 and first published on 26 Jun 2013


Article type: Paper
DOI: 10.1039/C3LC50374G
Citation: Lab Chip, 2013,13, 3747-3754
  •   Request permissions

    Flow-enhanced electrochemical immunosensors on centrifugal microfluidic platforms

    T. Kim, K. Abi-Samra, V. Sunkara, D. Park, M. Amasia, N. Kim, J. Kim, H. Kim, M. Madou and Y. Cho, Lab Chip, 2013, 13, 3747
    DOI: 10.1039/C3LC50374G

    If you are not the author of this article and you wish to reproduce material from it in a third party non-RSC publication you must formally request permission using RightsLink. Go to our Instructions for using RightsLink page for details.

    Authors contributing to RSC publications (journal articles, books or book chapters) do not need to formally request permission to reproduce material contained in this article provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      Reproduced from Ref. XX with permission from the Centre National de la Recherche Scientifique (CNRS) and The Royal Society of Chemistry.
    • For reproduction of material from PCCP:
      Reproduced from Ref. XX with permission from the PCCP Owner Societies.
    • For reproduction of material from PPS:
      Reproduced from Ref. XX with permission from the European Society for Photobiology, the European Photochemistry Association, and The Royal Society of Chemistry.
    • For reproduction of material from all other RSC journals and books:
      Reproduced from Ref. XX with permission from The Royal Society of Chemistry.

    If the material has been adapted instead of reproduced from the original RSC publication "Reproduced from" can be substituted with "Adapted from".

    In all cases the Ref. XX is the XXth reference in the list of references.

    If you are the author of this article you do not need to formally request permission to reproduce figures, diagrams etc. contained in this article in third party publications or in a thesis or dissertation provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Reproduced by permission of The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC
    • For reproduction of material from PCCP:
      [Original citation] - Reproduced by permission of the PCCP Owner Societies
    • For reproduction of material from PPS:
      [Original citation] - Reproduced by permission of The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC
    • For reproduction of material from all other RSC journals:
      [Original citation] - Reproduced by permission of The Royal Society of Chemistry

    If you are the author of this article you still need to obtain permission to reproduce the whole article in a third party publication with the exception of reproduction of the whole article in a thesis or dissertation.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements