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Issue 17, 2013
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Design of a prototype flow microreactor for synthetic biology in vitro

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Abstract

As a reference platform for in vitro synthetic biology, we have developed a prototype flow microreactor for enzymatic biosynthesis. We report the design, implementation, and computer-aided optimisation of a three-step model pathway within a microfluidic reactor. A packed bed format was shown to be optimal for enzyme compartmentalisation after experimental evaluation of several approaches. The specific substrate conversion efficiency could significantly be improved by an optimised parameter set obtained by computational modelling. Our microreactor design provides a platform to explore new in vitro synthetic biology solutions for industrial biosynthesis.

Graphical abstract: Design of a prototype flow microreactor for synthetic biology in vitro

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Publication details

The article was received on 19 Feb 2013, accepted on 27 Jun 2013 and first published on 27 Jun 2013


Article type: Paper
DOI: 10.1039/C3LC50231G
Citation: Lab Chip, 2013,13, 3426-3432
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    Design of a prototype flow microreactor for synthetic biology in vitro

    C. R. Boehm, P. S. Freemont and O. Ces, Lab Chip, 2013, 13, 3426
    DOI: 10.1039/C3LC50231G

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