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Issue 8, 2013
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A method for non-invasive full-field imaging and quantification of chemical species

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Abstract

We present a novel method for full-field scalar visualization and quantification of species concentration fields. We term this method species-altered fluorescence imaging (SAFI). The method employs electrically neutral fluorescent dyes whose quantum yields are selectively quenched or enhanced by species of interest. SAFI enables simultaneous imaging of material interfaces and provides non-invasive, scalar-field quantitation of two-dimensional species concentration fields. We describe criteria for choosing SAFI dyes and tabulate 35 promising SAFI dyes and their relevant properties. Next, we describe species concentration quantification with SAFI via Stern–Volmer quenching and discuss the sensitivity and resolution of our method. We demonstrate this method with two dyes, 6-methoxy-N-(3-sulfopropyl)quinolinium (SPQ) and 10-(3-sulfopropyl)acridinium betaine (SAB). We demonstrate our method in full-field visualization of several challenging electrokinetic flows: isotachophoresis (ITP) in both cationic and anionic modes, and in a convective electrokinetic instability (EKI) flow. Through these experiments we collectively quantify ion concentration shock velocities, simultaneously measure concentrations of five species, and quantify the development of an unsteady, chaotic, 2D flow.

Graphical abstract: A method for non-invasive full-field imaging and quantification of chemical species

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Publication details

The article was received on 23 Nov 2012, accepted on 29 Jan 2013 and first published on 30 Jan 2013


Article type: Paper
DOI: 10.1039/C3LC41293H
Citation: Lab Chip, 2013,13, 1632-1643
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    A method for non-invasive full-field imaging and quantification of chemical species

    V. Shkolnikov and J. G. Santiago, Lab Chip, 2013, 13, 1632
    DOI: 10.1039/C3LC41293H

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