Jump to main content
Jump to site search

Issue 9, 2013
Previous Article Next Article

Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples

Author affiliations

Abstract

On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38–40%) rate was 50 μl min−1 giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7–100 ng ml−1 within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%.

Graphical abstract: Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples

Back to tab navigation

Publication details

The article was received on 17 Nov 2012, accepted on 27 Feb 2013 and first published on 27 Feb 2013


Article type: Paper
DOI: 10.1039/C3LC41269E
Citation: Lab Chip, 2013,13, 1790-1796
  •   Request permissions

    Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples

    A. A. Tajudin, K. Petersson, A. Lenshof, A.-M. Swärd-Nilsson, L. Åberg, G. Marko-Varga, J. Malm, H. Lilja and T. Laurell, Lab Chip, 2013, 13, 1790
    DOI: 10.1039/C3LC41269E

Search articles by author

Spotlight

Advertisements