Jump to main content
Jump to site search

Issue 7, 2013
Previous Article Next Article

Four-part leukocyte differential count based on sheathless microflow cytometer and fluorescent dye assay

Author affiliations

Abstract

Leukocyte differential count is one of the most frequently ordered clinical tests in hospitals. This paper reports a point-of-care test for the leukocyte count by using a microflow cytometer and a fluorescent dye assay. The dye assay relied on fluorescent detection alone to count leukocytes in blood and to identify leukocyte subtypes. By combining the fluorescent assay with a sheathless microflow design, the proposed method achieved a minimal sample volume by eliminating excessive dilution and sheath flow. In this paper, a four-part leukocyte differential count including lymphocyte, monocyte, neutrophil and eosinophil was demonstrated, and the whole test consumed only a small amount of blood (5 μL) and reagents (68 μL in total). The merits of minimal sample volume, long reagent shelf life and portable instrument made this method optimal for point-of-care applications.

Graphical abstract: Four-part leukocyte differential count based on sheathless microflow cytometer and fluorescent dye assay

Back to tab navigation

Supplementary files

Publication details

The article was received on 19 Sep 2012, accepted on 17 Jan 2013 and first published on 18 Jan 2013


Article type: Paper
DOI: 10.1039/C3LC41059E
Citation: Lab Chip, 2013,13, 1257-1265
  •   Request permissions

    Four-part leukocyte differential count based on sheathless microflow cytometer and fluorescent dye assay

    W. Shi, L. Guo, H. Kasdan and Y. Tai, Lab Chip, 2013, 13, 1257
    DOI: 10.1039/C3LC41059E

Search articles by author

Spotlight

Advertisements