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Issue 9, 2013
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Optoacoustic tweezers: a programmable, localized cell concentrator based on opto-thermally generated, acoustically activated, surface bubbles

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Abstract

We present a programmable, biocompatible technique for dynamically concentrating and patterning particles and cells in a microfluidic device. Since our technique utilizes opto-thermally generated, acoustically activated, surface bubbles, we name it “optoacoustic tweezers”. The optoacoustic tweezers are capable of concentrating particles/cells at any prescribed locations in a microfluidic chamber without the use of permanent structures, rendering it particularly useful for the formation of flexible, complex cell patterns. Additionally, this technique has demonstrated excellent biocompatibility and can be conveniently integrated with other microfluidic units. In our experiments, micro-bubbles were generated by focusing a 405 nm diode laser onto a gold-coated glass chamber. By properly tuning the laser, we demonstrate precise control over the position and size of the generated bubbles. Acoustic waves were then applied to activate the surface bubbles, causing them to oscillate at an optimized frequency. The resulting acoustic radiation force allowed us to locally trap particles/cells, including 15 μm polystyrene beads and HeLa cells, around each bubble. Cell-adhesion tests were also conducted after cell concentrating to confirm the biocompatibility of this technique.

Graphical abstract: Optoacoustic tweezers: a programmable, localized cell concentrator based on opto-thermally generated, acoustically activated, surface bubbles

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Publication details

The article was received on 08 Jan 2013, accepted on 28 Feb 2013 and first published on 28 Feb 2013


Article type: Paper
DOI: 10.1039/C3LC00043E
Citation: Lab Chip, 2013,13, 1772-1779
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    Optoacoustic tweezers: a programmable, localized cell concentrator based on opto-thermally generated, acoustically activated, surface bubbles

    Y. Xie, C. Zhao, Y. Zhao, S. Li, J. Rufo, S. Yang, F. Guo and T. J. Huang, Lab Chip, 2013, 13, 1772
    DOI: 10.1039/C3LC00043E

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