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Issue 5, 2013
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Single-molecule mobility in confined and crowded femtolitre chambers

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Abstract

The effects of increased crowding and confinement on the mobility of individual fluorescent molecules were studied using Fluorescence Correlation Spectroscopy (FCS) in a microfluidic device with sealable femtolitre-volume chambers, and compared to three dimensional stochastic Monte Carlo simulations. When crowding and the degree of confinement were increased simultaneously, extended correlation times of fluorescent intensity fluctuations were observed with FCS compared to varying either crowding or confinement alone. Both experimental data and simulation suggest these extended correlation times were due to increased fluorophore adsorption–desorption events at the chamber lid in the presence of crowders. The data in increasingly confined and crowded chambers described here captures some of the salient features of crowding in cell-like environments.

Graphical abstract: Single-molecule mobility in confined and crowded femtolitre chambers

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Publication details

The article was received on 08 Aug 2012, accepted on 11 Dec 2012 and first published on 12 Dec 2012


Article type: Paper
DOI: 10.1039/C2LC40907K
Citation: Lab Chip, 2013,13, 877-885
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    Single-molecule mobility in confined and crowded femtolitre chambers

    J. D. Fowlkes and C. P. Collier, Lab Chip, 2013, 13, 877
    DOI: 10.1039/C2LC40907K

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