Issue 7, 2013

Development of a single-cell array for large-scale DNA fluorescence in situ hybridization

Abstract

DNA fluorescence in situ hybridization (FISH) is a powerful cytogenetic assay, but conventional sample-preparation methods for FISH do not support large-scale high-throughput data acquisition and analysis, which are potentially useful for several biomedical applications. To address this limitation, we have developed a novel FISH sample-preparation method based on generating a centimetre-sized cell array, in which all cells are precisely positioned and separated from their neighbours. This method is simple and capable of patterning nonadherent human cells. We have successfully performed DNA FISH on the single-cell arrays, which facilitates analysis of the FISH results with the FISH-FINDER computer program.

Graphical abstract: Development of a single-cell array for large-scale DNA fluorescence in situ hybridization

Supplementary files

Article information

Article type
Paper
Submitted
17 Apr 2012
Accepted
30 Nov 2012
First published
04 Dec 2012

Lab Chip, 2013,13, 1316-1324

Development of a single-cell array for large-scale DNA fluorescence in situ hybridization

Y. Liu, B. Kirkland, J. Shirley, Z. Wang, P. Zhang, J. Stembridge, W. Wong, S. Takebayashi, D. M. Gilbert, S. Lenhert and J. Guan, Lab Chip, 2013, 13, 1316 DOI: 10.1039/C2LC40364A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements