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Issue 10, 2013
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Site-specific peptide and protein immobilization on surface plasmon resonance chips via strain-promoted cycloaddition

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Abstract

Surface plasmon resonance (SPR) is a powerful label-free diagnostic tool to study biomolecular interactions. However, one of the drawbacks of SPR is the lack of controlled immobilization of ligands on the sensor surface. We have developed a modular platform for the fast, reagent-free and site-specific immobilization of azide-containing ligands by strain-promoted cycloaddition onto a cyclooctyne-modified SPR sensor surface. The usefulness of the concept was shown in a study with a papain model system, and up to 150 experiments were performed without loss of surface quality. Furthermore, azide-containing green fluorescent protein (GFP) was also effectively immobilized. Taken together, cyclooctyne-modified SPR chips enable smooth and site-selective immobilization of ligands and prove to be more robust than traditionally functionalized systems.

Graphical abstract: Site-specific peptide and protein immobilization on surface plasmon resonance chips via strain-promoted cycloaddition

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Publication details

The article was received on 06 Dec 2012, accepted on 12 Mar 2013 and first published on 12 Mar 2013


Article type: Paper
DOI: 10.1039/C3LC41338A
Citation: Lab Chip, 2013,13, 1863-1867
  • Open access: Creative Commons BY license
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    Site-specific peptide and protein immobilization on surface plasmon resonance chips via strain-promoted cycloaddition

    A. E. M. Wammes, M. J. E. Fischer, N. J. de Mol, M. B. van Eldijk, F. P. J. T. Rutjes, J. C. M. van Hest and F. L. van Delft, Lab Chip, 2013, 13, 1863
    DOI: 10.1039/C3LC41338A

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