Jump to main content
Jump to site search

Issue 19, 2013
Previous Article Next Article

Microfluidic electroporation for cellular analysis and delivery

Author affiliations

Abstract

Electroporation is a simple yet powerful technique for breaching the cell membrane barrier. The applications of electroporation can be generally divided into two categories: the release of intracellular proteins, nucleic acids and other metabolites for analysis and the delivery of exogenous reagents such as genes, drugs and nanoparticles with therapeutic purposes or for cellular manipulation. In this review, we go over the basic physics associated with cell electroporation and highlight recent technological advances on microfluidic platforms for conducting electroporation. Within the context of its working mechanism, we summarize the accumulated knowledge on how the parameters of electroporation affect its performance for various tasks. We discuss various strategies and designs for conducting electroporation at the microscale and then focus on analysis of intracellular contents and delivery of exogenous agents as two major applications of the technique. Finally, an outlook for future applications of microfluidic electroporation in increasingly diverse utilities is presented.

Graphical abstract: Microfluidic electroporation for cellular analysis and delivery

Back to tab navigation

Supplementary files

Publication details

The article was received on 07 May 2013, accepted on 06 Jul 2013 and first published on 08 Jul 2013


Article type: Critical Review
DOI: 10.1039/C3LC50566A
Citation: Lab Chip, 2013,13, 3803-3821
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Microfluidic electroporation for cellular analysis and delivery

    T. Geng and C. Lu, Lab Chip, 2013, 13, 3803
    DOI: 10.1039/C3LC50566A

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements