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Issue 14, 2013
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Sequential multi-molecule delivery using vortex-assisted electroporation

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Abstract

We developed an on-chip microscale electroporation system that enables sequential delivery of multiple molecules with precise and independent dosage controllability into pre-selected identical populations of target cells. The ability to trap cells with uniform size distribution contributed to enhanced molecular delivery efficiency and cell viability. Additionally, the system provides real-time monitoring ability of the entire delivery process, allowing timely and independent modification of cell- and molecule-specific electroporation parameters. The precisely controlled amount of inherently membrane-impermeant molecules was transferred into human cancer cells by varying electric field strengths and molecule injection durations. The proposed microfluidic electroporation system's improved viability and comparable gene transfection efficiency to that of commercial systems suggest that the current system has great potential to expand the research fields that on-chip electroporation techniques can be used in.

Graphical abstract: Sequential multi-molecule delivery using vortex-assisted electroporation

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Publication details

The article was received on 12 Feb 2013, accepted on 25 Apr 2013 and first published on 25 Apr 2013


Article type: Paper
DOI: 10.1039/C3LC50196E
Citation: Lab Chip, 2013,13, 2764-2772
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    Sequential multi-molecule delivery using vortex-assisted electroporation

    H. Yun and S. C. Hur, Lab Chip, 2013, 13, 2764
    DOI: 10.1039/C3LC50196E

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