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Issue 4, 2013
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Engineered cell culture substrates for axon guidance studies: moving beyond proof of concept

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Abstract

Promoting axon regeneration following injury is one of the ultimate challenges of neuroscience, and understanding the mechanisms that regulate axon growth and guidance is essential to achieve this goal. During development axons are directed over relatively long distances by a precise extracellular distribution of chemical signals in the embryonic nervous system. Multiple guidance proteins, including netrins, slits, semaphorins, ephrins and neurotrophins have been identified as key players in this process. During the last decade, engineered cell culture substrates have been developed to investigate the cellular and molecular mechanisms underlying axon guidance. This review is focused on the biological insights that have been achieved using new techniques that attempt to mimic in vitro the spatial patterns of proteins that growth cones encounter in vivo.

Graphical abstract: Engineered cell culture substrates for axon guidance studies: moving beyond proof of concept

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Publication details

The article was received on 31 Aug 2012, accepted on 09 Nov 2012 and first published on 12 Nov 2012


Article type: Critical Review
DOI: 10.1039/C2LC41002H
Citation: Lab Chip, 2013,13, 498-508
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    Engineered cell culture substrates for axon guidance studies: moving beyond proof of concept

    J. Roy, T. E. Kennedy and S. Costantino, Lab Chip, 2013, 13, 498
    DOI: 10.1039/C2LC41002H

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