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Issue 11, 2013
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A co-culture device with a tunable stiffness to understand combinatorial cell–cell and cell–matrix interactions

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Abstract

Cell behavior on 2-D in vitro cultures is continually being improved to better mimic in vivo physiological conditions by combining niche cues including multiple cell types and substrate stiffness, which are well known to impact cell phenotype. However, no system exists in which a user can systematically examine cell behavior on a substrate with a specific stiffness (elastic modulus) in culture with a different cell type, while maintaining distinct cell populations. We demonstrate the modification of a silicon reconfigurable co-culture system with a covalently linked hydrogel of user-defined stiffness. This device allows the user to control whether two separate cell populations are in contact with each other or only experience paracrine interactions on substrates of controllable stiffness. To illustrate the utility of this device, we examined the role of substrate stiffness combined with myoblast co-culture on adipose derived stem cell (ASC) differentiation and found that the presence of myoblasts and a 10 kPa substrate stiffness increased ASC myogenesis versus co-culture on stiff substrates. As this example highlights, this technology better controls the in vitro microenvironment, allowing the user to develop a more thorough understanding of the combined effects of cell–cell and cell–matrix interactions.

Graphical abstract: A co-culture device with a tunable stiffness to understand combinatorial cell–cell and cell–matrix interactions

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Publication details

The article was received on 23 Apr 2013, accepted on 07 Sep 2013 and first published on 13 Sep 2013


Article type: Paper
DOI: 10.1039/C3IB40078F
Citation: Integr. Biol., 2013,5, 1344-1354
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    A co-culture device with a tunable stiffness to understand combinatorial cell–cell and cell–matrix interactions

    N. Rao, G. N. Grover, L. G. Vincent, S. C. Evans, Y. S. Choi, K. H. Spencer, E. E. Hui, A. J. Engler and K. L. Christman, Integr. Biol., 2013, 5, 1344
    DOI: 10.1039/C3IB40078F

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