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Issue 6, 2013
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In vivo screening of extracellular matrix components produced under multiple experimental conditions implanted in one animal

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Abstract

Animal experiments help to progress and ensure safety of an increasing number of novel therapies, drug development and chemicals. Unfortunately, these also lead to major ethical concerns, costs and limited experimental capacity. We foresee a coercion of all these issues by implantation of well systems directly into vertebrate animals. Here, we used rapid prototyping to create wells with biomaterials to create a three-dimensional (3D) well-system that can be used in vitro and in vivo. First, the well sizes and numbers were adjusted for 3D cell culture and in vitro screening of molecules. Then, the functionality of the wells was evaluated in vivo under 36 conditions for tissue regeneration involving human mesenchymal stem cells (hMSCs) and bovine primary chondrocytes (bPCs) screened in one animal. Each biocompatible well was controlled to contain μl-size volumes of tissue, which led to tissue penetration from the host and tissue formation under implanted conditions. We quantified both physically and biologically the amounts of extracellular matrix (ECM) components found in each well. Using this new concept the co-culture of hMSCs and bPCs was identified as a positive hit for cartilage tissue repair, which was a comparable result using conventional methods. The in vivo screening of candidate conditions opens an entirely new range of experimental possibilities, which significantly abates experimental animal use and increases the pace of discovery of medical treatments.

Graphical abstract: In vivo screening of extracellular matrix components produced under multiple experimental conditions implanted in one animal

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Publication details

The article was received on 31 Jan 2013, accepted on 05 Apr 2013 and first published on 17 Apr 2013


Article type: Technical Innovation
DOI: 10.1039/C3IB40023A
Citation: Integr. Biol., 2013,5, 889-898
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    In vivo screening of extracellular matrix components produced under multiple experimental conditions implanted in one animal

    G. A. Higuera, J. A. A. Hendriks, J. van Dalum, L. Wu, R. Schotel, L. Moreira-Teixeira, M. van den Doel, J. C. H. Leijten, J. Riesle, M. Karperien, C. A. van Blitterswijk and L. Moroni, Integr. Biol., 2013, 5, 889
    DOI: 10.1039/C3IB40023A

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