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Issue 3, 2013
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Functional interplay between the cell cycle and cell phenotypes

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Cell cycle distribution of adherent cells is typically assessed using flow cytometry, which precludes the measurements of many cell properties and their cycle phase in the same environment. Here we develop and validate a microscopy system to quantitatively analyze the cell-cycle phase of thousands of adherent cells and their associated cell properties simultaneously. This assay demonstrates that population-averaged cell phenotypes can be written as a linear combination of cell-cycle fractions and phase-dependent phenotypes. By perturbing the cell cycle through inhibition of cell-cycle regulators or changing nuclear morphology by depletion of structural proteins, our results reveal that cell cycle regulators and structural proteins can significantly interfere with each other's prima facie functions. This study introduces a high-throughput method to simultaneously measure the cell cycle and phenotypes at single-cell resolution, which reveals a complex functional interplay between the cell cycle and cell phenotypes.

Graphical abstract: Functional interplay between the cell cycle and cell phenotypes

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The article was received on 14 Oct 2012, accepted on 05 Dec 2012 and first published on 14 Jan 2013

Article type: Paper
DOI: 10.1039/C2IB20246H
Citation: Integr. Biol., 2013,5, 523-534
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    Functional interplay between the cell cycle and cell phenotypes

    W. Chen, P. Wu, J. M. Phillip, S. B. Khatau, J. M. Choi, M. R. Dallas, K. Konstantopoulos, S. X. Sun, J. S. H. Lee, D. Hodzic and D. Wirtz, Integr. Biol., 2013, 5, 523
    DOI: 10.1039/C2IB20246H

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