Jump to main content
Jump to site search

Issue 1, 2013
Previous Article Next Article

Development of siRNA-probes for studying intracellular trafficking of siRNA nanoparticles

Author affiliations

Abstract

One important barrier facing the delivery of short interfering RNAs (siRNAs) via synthetic nanoparticles is the rate of nanoparticle disassembly. However, our ability to optimize the release kinetics of siRNAs from nanoparticles for maximum efficacy is limited by the lack of methods to track their intracellular disassembly. Towards this end, we describe the design of two different siRNA-based fluorescent probes whose fluorescence emission changes in response to the assembly state of the nanoparticle. The first probe design involves a redox-sensitive fluorescence-quenched probe that fluoresces only when the nanoparticle is disassembled in a reductive environment. The second probe design is based on a FRET-labeled siRNA pair that fluoresces due to the proximity of the siRNA pair when the nanoparticle is intact. In both approaches, the delivery vehicle need not be labeled. The utility of these probes was investigated with a lipidoid nanoparticle (LNP) as proof-of-concept in both extracellular and intracellular environments. Fluorescence kinetic data from both probes were fit to a two-phase release and decay curve and subsequently quantified to give intracellular disassembly rate constants. Quantitative analysis revealed that the rate constant of siRNA release measured via the fluorescence-quenched probe was almost identical to the rate constant for nanoparticle disassembly measured via the FRET-labeled probes. Furthermore, these probes were utilized to determine subcellular localization of LNPs with the use of automated high-resolution microscopy as they undergo dissociation. Interestingly, this work shows that large amounts of siRNA remain inside vesicular compartments. Altogether, we have developed new siRNA probes that can be utilized with multiple nanocarriers for quantitative and qualitative analysis of nanoparticle dissociation that may serve as a design tool for future delivery systems.

Graphical abstract: Development of siRNA-probes for studying intracellular trafficking of siRNA nanoparticles

Back to tab navigation

Publication details

The article was received on 21 Jun 2012, accepted on 24 Aug 2012 and first published on 29 Aug 2012


Article type: Technical Innovation
DOI: 10.1039/C2IB20155K
Citation: Integr. Biol., 2013,5, 224-230
  •   Request permissions

    Development of siRNA-probes for studying intracellular trafficking of siRNA nanoparticles

    C. A. Alabi, G. Sahay, R. Langer and D. G. Anderson, Integr. Biol., 2013, 5, 224
    DOI: 10.1039/C2IB20155K

Search articles by author

Spotlight

Advertisements