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Issue 8, 2013
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Evaluation of deep eutectic solventwater binary mixtures for lipase-catalyzed lipophilization of phenolic acids

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Abstract

This work reports the first lipase-catalyzed reactions between substrates of different polarities using deep eutectic solvents as a medium. The model reaction consisted of a lipophilization process based on the alcoholysis of phenolic esters using immobilized Candida antarctica lipase B as a biocatalyst. Results showed that water could dramatically improve the lipase activity and change the reactivity of phenolic substrates. Indeed, very low conversions (<2%) were observed in pure DES, whereas in DES–water binary mixtures, quantitative conversions were achieved. After investigating the role of various parameters, such as the substrate concentration and ratio, pH or thermodynamic activity of water, the effect of the presence of water in pure DES based on urea or glycerol was discussed. In this paper, we propose new perspectives for the enzymatic modification of polar substrates using this novel generation of green, inexpensive and easy-to-handle solvents.

Graphical abstract: Evaluation of deep eutectic solvent–water binary mixtures for lipase-catalyzed lipophilization of phenolic acids

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Publication details

The article was received on 15 May 2013, accepted on 24 Jun 2013 and first published on 24 Jun 2013


Article type: Paper
DOI: 10.1039/C3GC40899J
Citation: Green Chem., 2013,15, 2275-2282
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    Evaluation of deep eutectic solventwater binary mixtures for lipase-catalyzed lipophilization of phenolic acids

    E. Durand, J. Lecomte, B. Baréa, E. Dubreucq, R. Lortie and P. Villeneuve, Green Chem., 2013, 15, 2275
    DOI: 10.1039/C3GC40899J

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