Jump to main content
Jump to site search

Issue 2, 2013
Previous Article Next Article

Milk fat globule membrane isolate induces apoptosis in HT-29 human colon cancer cells

Author affiliations

Abstract

A native milk fat globule membrane (MFGM) isolate obtained from raw milk was assessed for its anticarcinogenic capacity using a colon cancer cell line (HT-29). To prevent microbial contamination and eliminate the presence of lipopolysaccharide (LPS) in the milk used for MFGM isolation, the milk was obtained from the mammary glands of cows using a catheter. Cell proliferation assays demonstrated a reduction of exponentially growing cancer cells of up to 53%, expressed as DNA synthesis (BrdU test), after 72 h stimulation with 100 μg of MFGM protein per mL. Using a similar MFGM concentration, the sulforhodamine B assay resulted in 57% reduction of cell density after 48 h incubation. This bioactivity was comparable to that of known anticancer drugs, 0.1 mM melphalan and 20 μM C2-ceramide, which achieved a cell division reduction of 25 and 40%, respectively, under the same experimental conditions. The toxic effect of the MFGM extracts on HT-29 cells was confirmed by the significant reduction in lactate dehydrogenase enzyme (LDH) by the residual viable cells. An increase of caspase-3 activity (up to 26%) led to the conclusion that MFGM has an apoptotic effect on HT-29 cancer cells.

Graphical abstract: Milk fat globule membrane isolate induces apoptosis in HT-29 human colon cancer cells

Back to tab navigation

Publication details

The article was received on 02 Aug 2012, accepted on 06 Oct 2012 and first published on 09 Oct 2012


Article type: Paper
DOI: 10.1039/C2FO30189J
Citation: Food Funct., 2013,4, 222-230
  •   Request permissions

    Milk fat globule membrane isolate induces apoptosis in HT-29 human colon cancer cells

    R. Zanabria, A. M. Tellez, M. Griffiths and M. Corredig, Food Funct., 2013, 4, 222
    DOI: 10.1039/C2FO30189J

Search articles by author

Spotlight

Advertisements