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Issue 2, 2014
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Preparation of hollow nickel silicate nanospheres for separation of His-tagged proteins

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Abstract

Hollow nickel silicate nanospheres (NiSiO3 NSs) with hierarchical shells were hydrothermally synthesized by using silica spheres as a template. The NiSiO3 NSs have an average diameter of 250 nm with a shell thickness of 50 nm, and the hierarchical shell consists of a large number of sheets. By taking advantage of the high affinity of Ni2+ toward histidine-tagged (His-tagged) proteins, hollow NiSiO3 NSs can be used to enrich and separate His-tagged proteins directly from a mixture of lysed cells. Results indicated that the hollow NiSiO3 NSs presented negligible nonspecific protein adsorption and a high protein binding ability with a high binding capacity of 13.2 mmol g−1. Their specificity and affinity toward His-tagged proteins remained after recycling 5 times. The hollow NiSiO3 NSs are especially suitable for rapid purification of His-tagged proteins.

Graphical abstract: Preparation of hollow nickel silicate nanospheres for separation of His-tagged proteins

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Publication details

The article was received on 01 Aug 2013, accepted on 25 Sep 2013 and first published on 25 Sep 2013


Article type: Paper
DOI: 10.1039/C3DT52084F
Citation: Dalton Trans., 2014,43, 779-783
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    Preparation of hollow nickel silicate nanospheres for separation of His-tagged proteins

    Y. Wu, G. Chang, Y. Zhao and Y. Zhang, Dalton Trans., 2014, 43, 779
    DOI: 10.1039/C3DT52084F

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