Biomimetic complexes are artificially engineered molecules that aim to reduce the structural complexity of biological systems in order to unveil the key electronic and structural factors relevant to a protein's function. In this work, a novel coordination compound (L₂Fe₂) which mimics non-heme binuclear proteins was synthesized from the Schiff-base ligand HL = (E)-N′-(phenyl(pyridin-2-yl)methylene)isonicotinohydrazide. The crystal structure of L₂Fe₂ showed that the intramolecular Fe–Fe distances (3.1–3.2 Å) were analogous to those found in non-heme binuclear ferric proteins. However, in L₂Fe₂, two methoxide groups act as bridging units for oxidized iron (Fe³⁺). Such a bridging motif is unprecedented in the biological realm. Magnetic susceptibility measurements demonstrated that L₂Fe₂ is characterized by a singlet (S = 0) ground state and a very small magnetic coupling constant J (≪ −1 cm⁻¹). The J value featured by L₂Fe₂ differs considerably from the values observed in non-heme binuclear proteins in the oxidized form (−100 cm⁻¹ < J < −10 cm⁻¹), which encompass oxo/hydroxo and carboxylate bridging residues. The singlet ground state of L₂Fe₂ as well as the weak magnetic interaction between the two ferric cations was successfully predicted by density functional theory (DFT).