Jump to main content
Jump to site search

Issue 6, 2013
Previous Article Next Article

Electro-enzymatic viologen-mediated substrate reduction using pentaerythritol tetranitrate reductase and a parallel, segmented fluid flow system

Author affiliations

Abstract

Many redox enzymes require expensive reduced cofactors like NAD(P)H which need to be recycled during catalysis, presenting a major cost and technical barrier to industrial exploitation. An electrochemical biphasic microfluidic setup is presented here, in which these cofactors are replaced by a mediator (methyl viologen) that acts by feeding electrons into the active site of the enzyme pentaerythritol tetranitrate reductase (PETNR). In this microfluidic recirculation setup, both enzyme and mediator remain in the reactor for reuse, allowing easy product recovery. System optimisation studies were performed using 2-cyclohexen-1-one as a model substrate prior to the investigation of a variety of different substrates whose reduction rates were determined to be 15–70% of those obtained when NADPH was used as sole electron donor. Additional data obtained with a thermophilic ‘ene’ reductase (TOYE) support the potential universality of this device for possible industrial applications.

Graphical abstract: Electro-enzymatic viologen-mediated substrate reduction using pentaerythritol tetranitrate reductase and a parallel, segmented fluid flow system

Back to tab navigation

Supplementary files

Publication details

The article was received on 23 Oct 2012, accepted on 23 Jan 2013 and first published on 05 Feb 2013


Article type: Paper
DOI: 10.1039/C3CY20720J
Citation: Catal. Sci. Technol., 2013,3, 1505-1511
  •   Request permissions

    Electro-enzymatic viologen-mediated substrate reduction using pentaerythritol tetranitrate reductase and a parallel, segmented fluid flow system

    K. Fisher, S. Mohr, D. Mansell, N. J. Goddard, P. R. Fielden and N. S. Scrutton, Catal. Sci. Technol., 2013, 3, 1505
    DOI: 10.1039/C3CY20720J

Search articles by author

Spotlight

Advertisements