Issue 5, 2013
From the journal:

Catalysis Science & Technology

Non-canonical amino acids as a useful synthetic biological tool for lipase-catalysed reactions in hostile environments†

Carlos G. Acevedo-Rocha,‡*ab   Michael G. Hoesl,‡c   Sebastian Nehring,‡c   Marina Royter,d   Christina Wolschner,c   Birgit Wiltschi,e   Garabed Antranikiand  and  Nediljko Budisac  

* Corresponding authors

a Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim, Germany
E-mail: acevedor@kofo.mpg.de

b Philipps-Universität Marburg, Fachbereich Chemie, Hans-Meerwein-Straße, 35032 Marburg, Germany
Tel: +49 (0)-6421-28-26082

c Berlin Institute of Technology, Department of Chemistry, Biocatalysis Group, Franklinstrasse 29, 10587 Berlin, Germany

d Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstrasse 12, 21073 Hamburg, Germany

e ACIB, Petersgasse 14, 8010 Graz, Austria

Abstract

The incorporation of several non-canonical amino acids into the Thermoanaerobacter thermohydrosulfuricus lipase confers not only activity enhancement upon treatment with organic solvents (by up to 450%) and surfactants (resp. 1630%), but also protective effects against protein reducing (resp. 140%), alkylating (resp. 160%), and denaturing (resp.190%) agents as well as inhibitors (resp. 40%). This approach offers novel chemically diversified biocatalysts for hostile environments.

Graphical abstract: Non-canonical amino acids as a useful synthetic biological tool for lipase-catalysed reactions in hostile environments

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Article information

DOI
https://doi.org/10.1039/C3CY20712A
Article type
Communication
Submitted
21 Oct 2012
Accepted
13 Feb 2013
First published
14 Feb 2013
This article is Open Access
Creative Commons BY-NC license

Catal. Sci. Technol., 2013,3, 1198-1201

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Non-canonical amino acids as a useful synthetic biological tool for lipase-catalysed reactions in hostile environments

C. G. Acevedo-Rocha, M. G. Hoesl, S. Nehring, M. Royter, C. Wolschner, B. Wiltschi, G. Antranikian and N. Budisa, Catal. Sci. Technol., 2013, 3, 1198 DOI: 10.1039/C3CY20712A

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