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Issue 15, 2013
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Whole-cell biocatalysis for selective and productive C–O functional group introduction and modification

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Abstract

During the last decades, biocatalysis became of increasing importance for chemical and pharmaceutical industries. Regarding regio- and stereospecificity, enzymes have shown to be superior compared to traditional chemical synthesis approaches, especially in C–O functional group chemistry. Catalysts established on a process level are diverse and can be classified along a functional continuum starting with single-step biotransformations using isolated enzymes or microbial strains towards fermentative processes with recombinant microorganisms containing artificial synthetic pathways. The complex organization of respective enzymes combined with aspects such as cofactor dependency and low stability in isolated form often favors the use of whole cells over that of isolated enzymes. Based on an inventory of the large spectrum of biocatalytic C–O functional group chemistry, this review focuses on highlighting the potentials, limitations, and solutions offered by the application of self-regenerating microbial cells as biocatalysts. Different cellular functionalities are discussed in the light of their (possible) contribution to catalyst efficiency. The combined achievements in the areas of protein, genetic, metabolic, and reaction engineering enable the development of whole-cell biocatalysts as powerful tools in organic synthesis.

Graphical abstract: Whole-cell biocatalysis for selective and productive C–O functional group introduction and modification

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Publication details

The article was received on 14 Jan 2013 and first published on 11 Mar 2013


Article type: Review Article
DOI: 10.1039/C3CS60011D
Citation: Chem. Soc. Rev., 2013,42, 6346-6377
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    Whole-cell biocatalysis for selective and productive C–O functional group introduction and modification

    M. Schrewe, M. K. Julsing, B. Bühler and A. Schmid, Chem. Soc. Rev., 2013, 42, 6346
    DOI: 10.1039/C3CS60011D

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