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Issue 43, 2013
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Sample preparation for single molecule localization microscopy

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Abstract

Single molecule localization-based optical nanoscopy was introduced in 2006, surpassing traditional diffraction-limited resolutions by an order of magnitude. Seven years later, this superresolution technique is continuing to follow a trend of increasing popularity and pervasiveness, with the proof-of-concept work long finished and commercial implementations now available. However one important aspect that tends to become lost in translation is the importance of proper sample preparation, with very few resources addressing the considerations that must be made when preparing samples for imaging with single molecule level sensitivity. Presented here is a an in-depth analysis of all aspects of sample preparation for single molecule superresolution, including both live and fixed cell preparation, choice of fluorophore, fixation and staining techniques, and imaging buffer considerations.

Graphical abstract: Sample preparation for single molecule localization microscopy

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Publication details

The article was received on 02 Sep 2013, accepted on 25 Sep 2013 and first published on 02 Oct 2013


Article type: Perspective
DOI: 10.1039/C3CP53719F
Citation: Phys. Chem. Chem. Phys., 2013,15, 18771-18783
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    Sample preparation for single molecule localization microscopy

    J. R. Allen, S. T. Ross and M. W. Davidson, Phys. Chem. Chem. Phys., 2013, 15, 18771
    DOI: 10.1039/C3CP53719F

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