Jump to main content
Jump to site search

Issue 14, 2013
Previous Article Next Article

Infrared multiple photon dissociation (IRMPD) spectroscopy of oxazine dyes

Author affiliations

Abstract

The structure and energetic properties of four common oxazine dyes, Nile red, Nile blue A, Cresyl violet, and Brilliant cresyl blue, have been probed using a combination of infrared multiple-photon dissociation (IRMPD) spectroscopy and quantum chemical calculations. IRMPD spectra of the protonated dyes, as generated from an electrospray ionization (ESI) source, were collected in the range of 900–1800 cm−1. Vibrational band assignments related to carbonyl and substituted-amine stretches were established from a comparison of the experimental spectra of these related systems as well as from a comparison with spectra generated by density functional theory (DFT) calculations. For Nile red, the thermochemical landscape for protonation at different basic sites was probed using DFT; comparison of IRMPD and calculated IR spectra reveals the site of protonation to be at the carbonyl oxygen. The structural information obtained here in the gas phase pertaining to these important fluorophores is anticipated to provide further insight into their associated intrinsic fluorescent properties in solution.

Graphical abstract: Infrared multiple photon dissociation (IRMPD) spectroscopy of oxazine dyes

Back to tab navigation

Publication details

The article was received on 24 Jun 2012, accepted on 06 Feb 2013 and first published on 06 Feb 2013


Article type: Paper
DOI: 10.1039/C3CP00158J
Citation: Phys. Chem. Chem. Phys., 2013,15, 5049-5056
  •   Request permissions

    Infrared multiple photon dissociation (IRMPD) spectroscopy of oxazine dyes

    R. J. Nieckarz, J. Oomens, G. Berden, P. Sagulenko and R. Zenobi, Phys. Chem. Chem. Phys., 2013, 15, 5049
    DOI: 10.1039/C3CP00158J

Search articles by author

Spotlight

Advertisements