Issue 84, 2013

Sandwich assay for mixed-sequence recognition of double-stranded DNA: Invader-based detection of targets specific to foodborne pathogens

Abstract

A 96-well plate sandwich assay based on Invader capture/signalling probes is used to recognize 28-mer mixed-sequence dsDNA targets specific to Salmonella enterica, Campylobacter jejuni, Escherichia coli. Targets are detected down to 20–55 pM concentration with excellent binding specificity.

Graphical abstract: Sandwich assay for mixed-sequence recognition of double-stranded DNA: Invader-based detection of targets specific to foodborne pathogens

Supplementary files

Article information

Article type
Communication
Submitted
26 Jul 2013
Accepted
09 Sep 2013
First published
09 Sep 2013

Chem. Commun., 2013,49, 9851-9853

Sandwich assay for mixed-sequence recognition of double-stranded DNA: Invader-based detection of targets specific to foodborne pathogens

B. Denn, S. Karmakar, D. C. Guenther and P. J. Hrdlicka, Chem. Commun., 2013, 49, 9851 DOI: 10.1039/C3CC45705B

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