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Issue 90, 2013
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Colorimetric detection of human papilloma virus by double isothermal amplification

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Abstract

We developed a polymerase reaction free, low-cost and sensitive assay for the colorimetric detection of Human Papilloma Virus (HPV), based on the use of a smart design exploiting magnetic microbeads, chimeric RNA/DNAzyme oligonucleotides, and double signal amplification. This method allows obtaining a fast response with a detection limit of 10 pM, avoiding the amplification of the target via traditional PCR.

Graphical abstract: Colorimetric detection of human papilloma virus by double isothermal amplification

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Publication details

The article was received on 18 Jul 2013, accepted on 22 Sep 2013 and first published on 23 Sep 2013


Article type: Communication
DOI: 10.1039/C3CC45459B
Citation: Chem. Commun., 2013,49, 10605-10607
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    Colorimetric detection of human papilloma virus by double isothermal amplification

    S. Persano, P. Valentini, J. H. Kim and P. P. Pompa, Chem. Commun., 2013, 49, 10605
    DOI: 10.1039/C3CC45459B

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