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Issue 65, 2013
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Backbone-modified molecular beacons for highly sensitive and selective detection of microRNAs based on duplex specific nuclease signal amplification

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Abstract

Based on backbone-modified molecular beacons and duplex-specific nuclease, we have developed a target recycling amplification method for highly sensitive and selective miRNA detection. The combination of a low fluorescence background of 2-OMe-RNA modified MB and nuclease-assisted signal amplification leads to ultrahigh assay sensitivity, and the powerful discriminating ability of MB enables the differentiation of highly similar miRNAs with one-base difference, both of which are of great significance to miRNA detection.

Graphical abstract: Backbone-modified molecular beacons for highly sensitive and selective detection of microRNAs based on duplex specific nuclease signal amplification

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Publication details

The article was received on 01 May 2013, accepted on 18 Jun 2013 and first published on 18 Jun 2013


Article type: Communication
DOI: 10.1039/C3CC43224F
Citation: Chem. Commun., 2013,49, 7243-7245
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    Backbone-modified molecular beacons for highly sensitive and selective detection of microRNAs based on duplex specific nuclease signal amplification

    X. Lin, C. Zhang, Y. Huang, Z. Zhu, X. Chen and C. J. Yang, Chem. Commun., 2013, 49, 7243
    DOI: 10.1039/C3CC43224F

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