Issue 77, 2013

Optical detection of epigenetic marks: sensitive quantification and direct imaging of individual hydroxymethylcytosine bases

Abstract

Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.

Graphical abstract: Optical detection of epigenetic marks: sensitive quantification and direct imaging of individual hydroxymethylcytosine bases

Supplementary files

Article information

Article type
Communication
Submitted
08 Apr 2013
Accepted
29 May 2013
First published
29 May 2013

Chem. Commun., 2013,49, 8599-8601

Optical detection of epigenetic marks: sensitive quantification and direct imaging of individual hydroxymethylcytosine bases

Y. Michaeli, T. Shahal, D. Torchinsky, A. Grunwald, R. Hoch and Y. Ebenstein, Chem. Commun., 2013, 49, 8599 DOI: 10.1039/C3CC42543F

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