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Issue 66, 2013
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Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention

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Abstract

The powerful strategy of “intracellular click reaction” was used to retain a chemical Ca2+ indicator in the cytosol. Specifically, a novel clickable Ca2+ indicator “N3-fura-2 AM” was coupled with dibenzylcyclooctyl-modified biomacromolecules via copper-free click reaction in living cells and Ca2+ oscillation was observed for an extended period of time.

Graphical abstract: Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention

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Publication details

The article was received on 06 Apr 2013, accepted on 16 May 2013 and first published on 16 May 2013


Article type: Communication
DOI: 10.1039/C3CC42489H
Citation: Chem. Commun., 2013,49, 7313-7315
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    Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention

    Y. Takei, A. Murata, K. Yamagishi, S. Arai, H. Nakamura, T. Inoue and S. Takeoka, Chem. Commun., 2013, 49, 7313
    DOI: 10.1039/C3CC42489H

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