Issue 50, 2013
From the journal:

Chemical Communications

Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe†

Ka-Ho Leung,‡a   Hong-Zhang He,‡a   Victor Pui-Yan Ma,a   Hai-Jing Zhong,b   Daniel Shiu-Hin Chan,a   Jun Zhou,cd   Jean-Louis Mergny,cd   Chung-Hang Leung*b  and  Dik-Lung Ma*a  

* Corresponding authors

a Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
E-mail: edmondma@hkbu.edu.hk

b State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
E-mail: duncanleung@umac.mo

c INSERM, U869, IECB, F-33600, Pessac, France

d Univ. Bordeaux, ARNA Laboratory, F-33000, Bordeaux, France

Abstract

We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(III) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated.

Graphical abstract: Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe

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Article information

DOI
https://doi.org/10.1039/C3CC41129J
Article type
Communication
Submitted
10 Feb 2013
Accepted
26 Mar 2013
First published
26 Mar 2013

Chem. Commun., 2013,49, 5630-5632

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Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe

K. Leung, H. He, V. P. Ma, H. Zhong, D. S. Chan, J. Zhou, J. Mergny, C. Leung and D. Ma, Chem. Commun., 2013, 49, 5630 DOI: 10.1039/C3CC41129J

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