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Issue 48, 2013
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A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres

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Abstract

We describe a simple, multiplexed assay that integrates glycan synthesis, bioconjugation to microspheres, fluorescent chemical/biochemical detection and multiparameter flow cytometric analysis to screen activities of different families of carbohydrate-active enzymes.

Graphical abstract: A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres

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Publication details

The article was received on 21 Dec 2012, accepted on 11 Mar 2013 and first published on 12 Mar 2013


Article type: Communication
DOI: 10.1039/C3CC39155H
Citation: Chem. Commun., 2013,49, 5441-5443
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    A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres

    A. Chandrasekaran, K. Deng, C. Koh, T. Takasuka, L. F. Bergeman, B. G. Fox, P. D. Adams and A. K. Singh, Chem. Commun., 2013, 49, 5441
    DOI: 10.1039/C3CC39155H

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