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Issue 98, 2013
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An improved fluorogenic NAD(P)+ detection method using 2-acetylbenzofuran: its origin and application

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Abstract

We have developed a fluorogenic NAD(P)+ detection method using 2-acetylbenzofuran. The reaction of NAD(P)+ with 2-acetylbenzofuran produced a fluorescent product, allowing the highly-sensitive and quick detection of NAD(P)+. This method was successfully applied to the detection of P450 substrates in the microtiter-plate format.

Graphical abstract: An improved fluorogenic NAD(P)+ detection method using 2-acetylbenzofuran: its origin and application

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Publication details

The article was received on 23 Sep 2013, accepted on 12 Oct 2013 and first published on 17 Oct 2013


Article type: Communication
DOI: 10.1039/C3CC47264G
Citation: Chem. Commun., 2013,49, 11500-11502
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    An improved fluorogenic NAD(P)+ detection method using 2-acetylbenzofuran: its origin and application

    T. Moriya, A. Kawamata, Y. Takahashi, Y. Iwabuchi and N. Kanoh, Chem. Commun., 2013, 49, 11500
    DOI: 10.1039/C3CC47264G

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