Division of Organic Chemistry, Institute of Chemical and Engineering Sciences, Agency for Science Technology and Research Singapore, 11 Biopolis Way, Helios Block #03-08, Singapore
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Chem. Commun., 2013,49, 11188-11190
05 Aug 2013,
11 Oct 2013
First published online
14 Oct 2013
Stabilized Wittig olefination holds great potential as a bioconjugation reaction. We demonstrate that the reaction of stabilized phosphorus ylides (or phosphonium salts) with aryl aldehydes is sufficiently robust to be used for live cell affinity isolation and fluorescence tagging of a protein, FKBP12.
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