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Issue 70, 2013
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An affinity capture involved enzymatic assay for thrombin by using peptide aptamers as affinity ligands on magnetic beads

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Abstract

Here we present a sensitive and specific assay for thrombin through the affinity capture of thrombin with mRNA display generated peptide aptamers on magnetic beads and the subsequent thrombin-involved enzymatic reaction. Thrombin at 50 fM can be detected.

Graphical abstract: An affinity capture involved enzymatic assay for thrombin by using peptide aptamers as affinity ligands on magnetic beads

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Publication details

The article was received on 11 Jun 2013, accepted on 02 Jul 2013 and first published on 02 Jul 2013


Article type: Communication
DOI: 10.1039/C3CC44400G
Citation: Chem. Commun., 2013,49, 7720-7722
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    An affinity capture involved enzymatic assay for thrombin by using peptide aptamers as affinity ligands on magnetic beads

    Q. Zhao and J. Gao, Chem. Commun., 2013, 49, 7720
    DOI: 10.1039/C3CC44400G

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