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Issue 77, 2013
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Optical detection of epigenetic marks: sensitive quantification and direct imaging of individual hydroxymethylcytosine bases

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Abstract

Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.

Graphical abstract: Optical detection of epigenetic marks: sensitive quantification and direct imaging of individual hydroxymethylcytosine bases

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Publication details

The article was received on 08 Apr 2013, accepted on 29 May 2013 and first published on 29 May 2013


Article type: Communication
DOI: 10.1039/C3CC42543F
Citation: Chem. Commun., 2013,49, 8599-8601
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    Optical detection of epigenetic marks: sensitive quantification and direct imaging of individual hydroxymethylcytosine bases

    Y. Michaeli, T. Shahal, D. Torchinsky, A. Grunwald, R. Hoch and Y. Ebenstein, Chem. Commun., 2013, 49, 8599
    DOI: 10.1039/C3CC42543F

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