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Issue 49, 2013
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Direct object resolution by image subtraction: a new molecular ruler for nanometric measurements on complexed fluorophores

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Abstract

A technique for measuring distances between two or more fluorophores spaced in the 10–100 nm range is described. We identify a linear correlation between the intensity–amplitude in the difference-image of single molecules undergoing fluorescence fluctuations and their separation. The transform is used to map distances between coupled fluorophores.

Graphical abstract: Direct object resolution by image subtraction: a new molecular ruler for nanometric measurements on complexed fluorophores

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Publication details

The article was received on 21 Mar 2013, accepted on 02 May 2013 and first published on 03 May 2013


Article type: Communication
DOI: 10.1039/C3CC42072H
Citation: Chem. Commun., 2013,49, 5559-5561
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    Direct object resolution by image subtraction: a new molecular ruler for nanometric measurements on complexed fluorophores

    R. L. Boulineau and M. A. Osborne, Chem. Commun., 2013, 49, 5559
    DOI: 10.1039/C3CC42072H

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