Issue 49, 2013
From the journal:

Chemical Communications

Direct object resolution by image subtraction: a new molecular ruler for nanometric measurements on complexed fluorophores†

Rémi L. Boulineaua  and  Mark A. Osborne*a  

* Corresponding authors

a Department of Chemistry, School of Life Sciences, University of Sussex, Falmer, UK
E-mail: m.osborne@sussex.ac.uk
Fax: +44 (0)1273 78323
Tel: +44 (0)1273 678328

Abstract

A technique for measuring distances between two or more fluorophores spaced in the 10–100 nm range is described. We identify a linear correlation between the intensity–amplitude in the difference-image of single molecules undergoing fluorescence fluctuations and their separation. The transform is used to map distances between coupled fluorophores.

Graphical abstract: Direct object resolution by image subtraction: a new molecular ruler for nanometric measurements on complexed fluorophores

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Article information

DOI
https://doi.org/10.1039/C3CC42072H
Article type
Communication
Submitted
21 Mar 2013
Accepted
02 May 2013
First published
03 May 2013

Chem. Commun., 2013,49, 5559-5561

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Direct object resolution by image subtraction: a new molecular ruler for nanometric measurements on complexed fluorophores

R. L. Boulineau and M. A. Osborne, Chem. Commun., 2013, 49, 5559 DOI: 10.1039/C3CC42072H

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