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Issue 51, 2013
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Rapid electrochemical screening of NAD-dependent dehydrogenases in a 96-well format

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Abstract

The electrochemical detection of dehydrogenase activity in crude cell lysates is performed simultaneously using 96 carbon electrodes modified with electrografted phenazines. The method is applied to the screening of a library of formate dehydrogenase mutants obtained by directed evolution.

Graphical abstract: Rapid electrochemical screening of NAD-dependent dehydrogenases in a 96-well format

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Publication details

The article was received on 21 Mar 2013, accepted on 30 Apr 2013 and first published on 01 May 2013


Article type: Communication
DOI: 10.1039/C3CC42065E
Citation: Chem. Commun., 2013,49, 5781-5783
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    Rapid electrochemical screening of NAD-dependent dehydrogenases in a 96-well format

    S. Abdellaoui, M. Bekhouche, A. Noiriel, R. Henkens, C. Bonaventura, L. J. Blum and B. Doumèche, Chem. Commun., 2013, 49, 5781
    DOI: 10.1039/C3CC42065E

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