Issue 49, 2013
From the journal:

Chemical Communications

A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides†

Shujiro Shiosaki,‡a   Takanobu Nobori,‡a   Takeshi Mori,bc   Riki Toita,a   Yuta Nakamura,a   Chan Woo Kim,a   Tatsuhiro Yamamoto,d   Takuro Niidomebc  and  Yoshiki Katayama*bcef  

* Corresponding authors

a Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan

b Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan
E-mail: ykatatcm@mail.cstm.kyushu-u.ac.jp
Fax: +81 92-802-2850
Tel: +81 92-802-2850

c Center for Future Chemistry, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan

d Institute of Systems, Information Technologies and Nanotechnologies, 203-1 Motooka, Nishi-Ku, Fukuoka 819-0385, Japan

e International Research Center for Molecular Systems, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan

f Center for Advanced Medical Innovation, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan

Abstract

A novel protein kinase assay was developed, based on FRET between QDs and fluorescently-labeled substrate peptides. The negatively charged QDs recognize the change in net charge of the peptide upon phosphorylation. Despite its simple mechanism, this assay is sensitive and robust enough to be applied to the evaluation of protein kinase inhibitors.

Graphical abstract: A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C3CC41680A
Article type
Communication
Submitted
06 Mar 2013
Accepted
29 Apr 2013
First published
30 Apr 2013

Chem. Commun., 2013,49, 5592-5594

Permissions

Request permissions

A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides

S. Shiosaki, T. Nobori, T. Mori, R. Toita, Y. Nakamura, C. W. Kim, T. Yamamoto, T. Niidome and Y. Katayama, Chem. Commun., 2013, 49, 5592 DOI: 10.1039/C3CC41680A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements