Endonuclease IV discriminates mismatches next to the apurinic/apyrimidinic site in DNA strands: constructing DNA sensing platforms with extremely high selectivity

Xianjin Xiao; Yang Liu; Meiping Zhao

doi:10.1039/C3CC40902C

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Chemical Communications

Issue 27, 2013

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Endonuclease IV discriminates mismatches next to the apurinic/apyrimidinic site in DNA strands: constructing DNA sensing platforms with extremely high selectivity

Xianjin Xiao,^a Yang Liu^a and Meiping Zhao*^a

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Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, China
E-mail: mpzhao@pku.edu.cn ;
Fax: +86-10-62751708

Chem. Commun., 2013,49, 2819-2821

DOI: 10.1039/C3CC40902C
Received 02 Feb 2013, Accepted 18 Feb 2013
First published online 19 Feb 2013

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A unique capability of Endonuclease IV in discrimination of mismatches neighboring a natural abasic site in DNA strands has been demonstrated, which enables genotyping of SNPs with high discrimination factors and differentiation of as low as 0.1–0.01% of target DNA strands from a large background of single-base different interfering strands.

Abstract from Analytical Abstracts 2013

A unique capability of Endonuclease IV in discrimination of mismatches neighboring a natural abasic site in DNA strands has been demonstrated, which enables genotyping of SNPs with high discrimination factors and differentiation of as low as 0.1-0.01% of target DNA strands from a large background of single-base different interfering strands.

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Chemical Communications

Endonuclease IV discriminates mismatches next to the apurinic/apyrimidinic site in DNA strands: constructing DNA sensing platforms with extremely high selectivity

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Endonuclease IV discriminates mismatches next to the apurinic/apyrimidinic site in DNA strands: constructing DNA sensing platforms with extremely high selectivity

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