A click-activated fluorescent probe for selective detection of hydrazoic acid and its application in biological imaging

Yi Zhou; Yue-Wei Yao; Qi Qi; Yuan Fang; Jing-Yun Li; Cheng Yao

doi:10.1039/C3CC40698A

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Chemical Communications

Issue 53, 2013

Urgent high quality communications from across the chemical sciences.

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A click-activated fluorescent probe for selective detection of hydrazoic acid and its application in biological imaging

Yi Zhou,^a Yue-Wei Yao,^a^b Qi Qi,^b Yuan Fang,^a Jing-Yun Li^c and Cheng Yao*^a

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State Key Laboratory of Materials-Oriented Chemical Engineering and College of Science, Nanjing University of Technology, Nanjing 210009, P. R. China
E-mail: yaochengnjut@126.com;
Fax: +86-25-8358-7433

College of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, P. R. China

Model Animal Research Center, Nanjing University, Nanjing 210061, P. R. China

Chem. Commun., 2013,49, 5924-5926

DOI: 10.1039/C3CC40698A
Received 27 Jan 2013, Accepted 09 Mar 2013
First published online 13 Mar 2013

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Here we present a new approach to aqueous hydrazoic acid detection through the synthesis and evaluation of an alkyne-based fluorescent probe, which could be applied in the monitoring of hydrazoic acid in both living Hela cells and larval zebrafish. This probe can also serve as an early warning automaton which would alert when both azide and protons exist over the threshold value.