Issue 23, 2013
From the journal:

Chemical Communications

Efficient chemical synthesis of human complement protein C3a†

Artin Ghassemian,‡§a   Ching-I Anderson Wang,§a   Mei-Kwan Yau,a   Robert C. Reid,a   Richard J. Lewis,a   David P. Fairlie,a   Paul F. Alewooda  and  Thomas Durek*a  

* Corresponding authors

a Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St Lucia, Brisbane, Queensland, Australia
E-mail: thomas.durek@gmail.com
Fax: +61-7-3346-2090
Tel: +61-7-3346-2985

Abstract

We report the total chemical synthesis of human C3a by one-pot native chemical ligation of three unprotected peptide segments, followed by efficient in vitro folding that yielded the anaphylatoxin C3a in high yield and excellent purity. Synthetic C3a was fully active and its crystal structure at 2.1 Å resolution showed 3 helices and a C-terminal turn motif.

Graphical abstract: Efficient chemical synthesis of human complement protein C3a

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Article information

DOI
https://doi.org/10.1039/C3CC40537K
Article type
Communication
Submitted
22 Jan 2013
Accepted
06 Feb 2013
First published
08 Feb 2013

Chem. Commun., 2013,49, 2356-2358

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Efficient chemical synthesis of human complement protein C3a

A. Ghassemian, C. A. Wang, M. Yau, R. C. Reid, R. J. Lewis, D. P. Fairlie, P. F. Alewood and T. Durek, Chem. Commun., 2013, 49, 2356 DOI: 10.1039/C3CC40537K

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