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Issue 27, 2013
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A universal and multiplex kinase assay using γ-[18O4]-ATP

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Abstract

We have synthesized γ-[18O4]-ATP and used it to develop a non-radioactive and multiplex method. Significantly, this novel approach can be applied to any kinases without using a purified enzyme or a fluorescent substrate. Using this approach, the effectiveness and specificity of inhibitors on several kinases could be readily determined.

Graphical abstract: A universal and multiplex kinase assay using γ-[18O4]-ATP

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Publication details

The article was received on 25 Nov 2012, accepted on 16 Jan 2013 and first published on 18 Jan 2013


Article type: Communication
DOI: 10.1039/C3CC38467E
Citation: Chem. Commun., 2013,49, 2795-2797
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    A universal and multiplex kinase assay using γ-[18O4]-ATP

    C. Fu, X. Zheng, Y. Jiang, Y. Liu, P. Xu, Z. Zeng, R. Liu and Y. Zhao, Chem. Commun., 2013, 49, 2795
    DOI: 10.1039/C3CC38467E

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