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Issue 36, 2013
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Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis

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Abstract

Peptide nucleic acid (PNA) probes were designed to bind to the internal reference sequence and the single base mutation sequence within PCR-amplified DNA templates. PNAs hybridized to the target sequences on DNA were analyzed using MALDI-TOF mass spectrometry. Accurate quantification of the relative amount of mutant DNA was reproducibly demonstrated.

Graphical abstract: Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis

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Publication details

The article was received on 04 Jan 2013, accepted on 18 Mar 2013 and first published on 18 Mar 2013


Article type: Communication
DOI: 10.1039/C3CC00070B
Citation: Chem. Commun., 2013,49, 3754-3756
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    Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis

    Y. J. Yeo, K. Roh, J. Y. Bang, E. H. Lee, H. S. Park and D. Kim, Chem. Commun., 2013, 49, 3754
    DOI: 10.1039/C3CC00070B

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