Rational design of Hg2+ controlled streptavidin-binding aptamer

Hongcheng Mei; Tao Bing; Cui Qi; Nan Zhang; Xiangjun Liu; Tianjun Chang; Jingli Yan; Dihua Shangguan

doi:10.1039/C2CC36416F

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Rational design of Hg²⁺ controlled streptavidin-binding aptamer†

Hongcheng Mei,^ab Tao Bing,^a Cui Qi,^ab Nan Zhang,^ab Xiangjun Liu,^a Tianjun Chang,^ab Jingli Yan^ab and Dihua Shangguan*^a

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* Corresponding authors

^a Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China
E-mail: sgdh@iccas.ac.cn
Fax: +86-10-62528509
Tel: +86-10-62528509

^b Graduate School of the Chinese Academy of Sciences, Beijing, China

Abstract

Through substituting two canonical base pairs of a streptavidin-binding aptamer with T–T mismatched base pairs, a new aptamer was constructed. Its binding ability could be controlled by Hg²⁺ through the formation of T–Hg–T metal–base pairs.

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Article information

DOI: https://doi.org/10.1039/C2CC36416F
Article type: Communication
Submitted: 03 Sep 2012
Accepted: 07 Nov 2012
First published: 08 Nov 2012

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Chem. Commun., 2013,49, 164-166

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Rational design of Hg²⁺ controlled streptavidin-binding aptamer

H. Mei, T. Bing, C. Qi, N. Zhang, X. Liu, T. Chang, J. Yan and D. Shangguan, Chem. Commun., 2013, 49, 164 DOI: 10.1039/C2CC36416F

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