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Issue 7, 2013
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Cationic, helical polypeptide-based gene delivery for IMR-90 fibroblasts and human embryonic stem cells

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Abstract

Diblock copolymers consisting of poly(ethylene glycol)-block-poly(γ-4-(((2-(piperidin-1-yl)ethyl)amino)methyl)benzyl-L-glutamate) (PEG-b-PVBLG-8) were synthesized and evaluated for their ability to mediate gene delivery in hard-to-transfect cells like IMR-90 human fetal lung fibroblasts and human embryonic stem cells (hESCs). The PEG-b-PVBLG-8 contained a membrane-disruptive, cationic, helical polypeptide block (PVBLG-8) for complexing with DNA and a hydrophilic PEG block to improve the biocompatibility of the gene delivery vehicle. The incorporation of PEG effectively reduced the toxicity of the helical PVBLG-8 block without dramatically compromising the polymer's ability to destabilize membranes or form complexes with DNA. PEG-b-PVBLG-8 copolymers with low (n = 76) and high (n = 287) degrees of polymerization (n) of the PVBLG-8 block were synthesized and evaluated for gene delivery. PEG-b-PVBLG-8 diblock polymers with a high degree of polymerization have a greater transfection efficiency and lower toxicity in IMR-90 cells than the commercial reagent Lipofectamine 2000. The usefulness of PEG-b-PVBLG-8 was further demonstrated via the successful transfection of hESCs without a measured loss in cell pluripotency markers.

Graphical abstract: Cationic, helical polypeptide-based gene delivery for IMR-90 fibroblasts and human embryonic stem cells

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Publication details

The article was received on 04 Jan 2013, accepted on 19 Mar 2013 and first published on 08 Apr 2013


Article type: Paper
DOI: 10.1039/C3BM00006K
Citation: Biomater. Sci., 2013,1, 719-727
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    Cationic, helical polypeptide-based gene delivery for IMR-90 fibroblasts and human embryonic stem cells

    J. Yen, Y. Zhang, N. P. Gabrielson, L. Yin, L. Guan, I. Chaudhury, H. Lu, F. Wang and J. Cheng, Biomater. Sci., 2013, 1, 719
    DOI: 10.1039/C3BM00006K

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