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Issue 23, 2013
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Supercritical fluid chromatography-tandem mass spectrometry for the analysis of lipid A

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Abstract

Although supercritical fluid chromatography (SFC) is often useful for the analysis of nonpolar lipids, this study demonstrates for the first time an analytical SFC method with detection by ion trap mass spectrometry (MS/MS) for large and polar lipids, such as lipid A, using a simple, fast, economical, and environmentally-friendly approach. Chemical derivatization of the lipid A was not needed, and the SFC-MS/MS analysis only took 2 min per injection. The method was evaluated by analyzing lipid A spiked into two representative food matrices, lettuce and ground beef. For sample preparation, a simple shaking procedure with 3/1 (v/v) chloroform–methanol was used. %Recoveries in spiked samples were 74 ± 10 at 10–30 μg g−1 in lettuce and 110 ± 5 in meat at 30 μg g−1.

Graphical abstract: Supercritical fluid chromatography-tandem mass spectrometry for the analysis of lipid A

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Publication details

The article was received on 05 Aug 2013, accepted on 16 Sep 2013 and first published on 27 Sep 2013


Article type: Technical Note
DOI: 10.1039/C3AY41344F
Citation: Anal. Methods, 2013,5, 6864-6869
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    Supercritical fluid chromatography-tandem mass spectrometry for the analysis of lipid A

    Y. Chen, S. J. Lehotay and R. A. Moreau, Anal. Methods, 2013, 5, 6864
    DOI: 10.1039/C3AY41344F

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