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Issue 23, 2013
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Fluorescence spectroscopy and multi-way techniques. PARAFAC

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Abstract

PARAllel FACtor analysis (PARAFAC) is increasingly used to decompose fluorescence excitation emission matrices (EEMs) into their underlying chemical components. In the ideal case where fluorescence conforms to Beers Law, this process can lead to the mathematical identification and quantification of independently varying fluorophores. However, many practical and analytical hurdles stand between EEM datasets and their chemical interpretation. This article provides a tutorial in the practical application of PARAFAC to fluorescence datasets, demonstrated using a dissolved organic matter (DOM) fluorescence dataset. A new toolbox for MATLAB is presented to support improved visualisation and sensitivity analyses of PARAFAC models in fluorescence spectroscopy.

Graphical abstract: Fluorescence spectroscopy and multi-way techniques. PARAFAC

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Publication details

The article was received on 12 Jul 2013, accepted on 09 Sep 2013 and first published on 10 Sep 2013


Article type: Tutorial Review
DOI: 10.1039/C3AY41160E
Citation: Anal. Methods, 2013,5, 6557-6566
  • Open access: Creative Commons BY-NC license
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    Fluorescence spectroscopy and multi-way techniques. PARAFAC

    K. R. Murphy, C. A. Stedmon, D. Graeber and R. Bro, Anal. Methods, 2013, 5, 6557
    DOI: 10.1039/C3AY41160E

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